Abstract
The genomes of several nonphotosynthetic bacteria, such as Bacillus subtilis, and some Archaea include genes for proteins with sequence homology to the large subunit of ribulose bisphosphate carboxylase/oxygenase (RuBisCO). We found that such a RuBisCO-like protein (RLP) from B. subtilis catalyzed the 2,3-diketo-5-methylthiopentyl-1-phosphate enolase reaction in the methionine salvage pathway. A growth-defective mutant, in which the gene for this RLP had been disrupted, was rescued by the gene for RuBisCOfrom the photosynthetic bacterium Rhodospirillum rubrum. Thus, the photosynthetic RuBisCO from R. rubrum retains the ability to function in the methionine salvage pathway in B. subtilis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology*
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Bacillus subtilis / genetics
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Bacillus subtilis / growth & development
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Catalysis
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Genes, Bacterial
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Magnetic Resonance Spectroscopy
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Methionine / metabolism
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Molecular Sequence Data
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Mutation
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Operon
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Phylogeny
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Recombinant Proteins / metabolism
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Rhodospirillum rubrum / enzymology*
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Rhodospirillum rubrum / genetics
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Ribulose-Bisphosphate Carboxylase / chemistry
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Ribulose-Bisphosphate Carboxylase / genetics
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Ribulose-Bisphosphate Carboxylase / metabolism*
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Sequence Alignment
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Thioglycosides / metabolism
Substances
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Bacterial Proteins
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Recombinant Proteins
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Thioglycosides
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5-methylthioribose
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Methionine
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Ribulose-Bisphosphate Carboxylase