[Effect of human telomerase reverse transcriptase mRNA introduction on the growth of human umbilicus vein endothelial cell]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2003 Sep;17(5):411-3.
[Article in Chinese]

Abstract

Objective: To elongate the proliferation life-span of human umbilicus vein endothelial cell (HUVEC).

Methods: We synthesized the human telomerase reverse transcriptase mRNA (hTERT mRNA) by in vitro transcription, then transferred the hTERT mRNA into HUVEC in quicent stage by lipofect introduction.

Results: Telomerase expressed transiently in HUVEC, and the cell life-span was elongated for 7 population doublings.

Conclusion: Telomerase can be reconstructed controllably and transiently in HUVEC by hTERT mRNA introduction, this method has the potential to be used to elongate the lifespan of cells cultured in vitro.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Cells, Cultured
  • DNA-Binding Proteins
  • Endothelium, Vascular / cytology*
  • Humans
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Telomerase* / biosynthesis
  • Telomerase* / genetics
  • Telomerase* / metabolism
  • Transfection*
  • Umbilical Veins / cytology

Substances

  • DNA-Binding Proteins
  • RNA, Messenger
  • Telomerase