[Detection of platelet activation by flow cytometry in patients with pre-eclampsia]

Di Yi Jun Yi Da Xue Xue Bao. 2003 Oct;23(10):1095-6.
[Article in Chinese]

Abstract

Objective: To explore the clinical significance of expression alterations of static and activated platelet glycoprotein CD41a, CD62p, and CD63 in patients with pre-eclampsia.

Methods: We used non-washing immunolabeling technique for fluorescence labeling of the static and activated platelet with CD41a, CD62p, and CD63 monoclonal antibodies of the blood samples from 16 patients with pre-eclampsia, 15 women with normal pregnancies and 20 healthy subjects. The expression of the monoclonal antibody was detected by flow cytometry.

Results: The average static and activated platelet glycoprotein CD41a, CD62p, and CD63 expression levels in patients with pre-eclampsia were apparently higher than those of the normal pregnant women (P<0.01), while the comparison between the latter and the control group did not reveal any significant differences (P>0.05).

Conclusion: High levels of platelet glycoprotein CD62p and CD63 expressions are present in patients with preeclampsia, suggesting the presence of platelet activation in these patients.

MeSH terms

  • Adult
  • Antigens, CD / blood
  • Blood Platelets / chemistry
  • Female
  • Flow Cytometry / methods*
  • Humans
  • P-Selectin / blood
  • Platelet Activation*
  • Platelet Membrane Glycoprotein IIb / blood
  • Platelet Membrane Glycoproteins
  • Pre-Eclampsia / blood*
  • Pregnancy
  • Tetraspanin 30

Substances

  • Antigens, CD
  • CD63 protein, human
  • P-Selectin
  • Platelet Membrane Glycoprotein IIb
  • Platelet Membrane Glycoproteins
  • Tetraspanin 30