Ly49D receptor expressed on immature B cells regulates their IFN-gamma secretion, actin polymerization, and homing

J Immunol. 2003 Nov 1;171(9):4630-8. doi: 10.4049/jimmunol.171.9.4630.

Abstract

Low levels of IFN-gamma secreted by immature B cells prevent their own migration and homing to the lymph nodes and premature encounter with Ag. In this study we followed the mechanism regulating IFN-gamma secretion by immature B cells. We show that the MHC class I receptor, Ly49D, is expressed on immature B cells and is down-regulated during maturation. Activation of this receptor leads to increase in IFN-gamma transcription and translation and results in the altered ability of B cells to polymerize actin in response to chemokine stimulation. Moreover, we show that H2-D blockage inhibits the ability of immature B cells to transcribe the IFN-gamma gene and results in rescue of cytoskeletal rearrangement. Thus, Ly49D that is expressed on immature B cells recognizes MHC class I on the peripheral tissues, inducing the secretion of low levels of IFN-gamma and thereby down-regulating immature B cell homing to the lymph nodes or to sites of inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Animals
  • Antibodies, Blocking / pharmacology
  • Antigens, Ly / biosynthesis
  • Antigens, Ly / physiology*
  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocyte Subsets / metabolism*
  • Cell Differentiation / immunology
  • Cell Line, Tumor
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cell Movement / genetics
  • Cell Movement / immunology*
  • H-2 Antigens / genetics
  • H-2 Antigens / immunology
  • H-2 Antigens / physiology
  • Histocompatibility Antigen H-2D
  • Interferon-gamma / antagonists & inhibitors
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / metabolism*
  • Lectins, C-Type
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NK Cell Lectin-Like Receptor Subfamily A
  • Protein Biosynthesis / immunology
  • Receptors, Lymphocyte Homing / biosynthesis
  • Receptors, Lymphocyte Homing / physiology*
  • Receptors, NK Cell Lectin-Like
  • Transcription, Genetic / immunology
  • Up-Regulation / genetics
  • Up-Regulation / immunology

Substances

  • Actins
  • Antibodies, Blocking
  • Antigens, Ly
  • H-2 Antigens
  • H-2Kb protein, mouse
  • Histocompatibility Antigen H-2D
  • Klra4 protein, mouse
  • Lectins, C-Type
  • NK Cell Lectin-Like Receptor Subfamily A
  • Receptors, Lymphocyte Homing
  • Receptors, NK Cell Lectin-Like
  • Interferon-gamma