Measurement of the levels of polymerized actin (F-actin) in chemokine-stimulated lymphocytes and GFP-coupled cDNA transfected lymphoid cells by flow cytometry

Methods Mol Biol. 2004:239:53-68. doi: 10.1385/1-59259-435-2:53.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / analysis*
  • Actins / metabolism
  • Chemokines / pharmacology*
  • Chemotaxis, Leukocyte / drug effects
  • Chemotaxis, Leukocyte / physiology
  • DNA, Complementary / genetics
  • Flow Cytometry / methods*
  • Green Fluorescent Proteins
  • Humans
  • In Vitro Techniques
  • Jurkat Cells
  • Luminescent Proteins / genetics
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism*
  • Plasmids / genetics
  • Recombinant Proteins / genetics
  • Transfection

Substances

  • Actins
  • Chemokines
  • DNA, Complementary
  • Luminescent Proteins
  • Recombinant Proteins
  • Green Fluorescent Proteins