Distribution and configuration of nucleolar DNA in the nucleolus of pea (Pisum sativum) were analyzed using cytochemical NAMA-Ur DNA specific stain method. The results showed that nucleolar DNA distributed in FC and juncture of FC/DFC in the forms of surrounding FC. rDNA in different positions existed in either condensed or uncondensed forms. Nucleolus-associated chromatin outside nucleolus extended in the periphery of FC via channels in the nucleolus.