Abstract
Expression of the rat peroxisomal 3-ketoacyl-CoA thiolase gene B is induced by peroxisome proliferators. Although a sequence element like a peroxisome proliferator-activated receptor (PPAR)-binding site is located in the promoter region of this gene, we previously found that this element is competent for the activation by hepatocyte nuclear factor-4, but not functional with PPARalpha. We describe here a new peroxisome proliferator-response element located in the intron 3 (+1422/+1434) that binds in vitro the PPARalpha/retinoid X receptor alpha heterodimer and confers the induction by PPARalpha in transfection assays. We propose a model of regulation of the rat thiolase B gene involving those elements in the promoter and intron 3.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl-CoA C-Acetyltransferase / genetics*
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Acetyl-CoA C-Acetyltransferase / metabolism*
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Animals
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Cells, Cultured
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DNA-Binding Proteins*
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Gene Expression Regulation, Enzymologic / physiology*
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Hepatocyte Nuclear Factor 4
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Introns
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Liver / enzymology*
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Peroxisome Proliferators / metabolism
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Peroxisomes / enzymology*
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Peroxisomes / genetics*
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Phosphoproteins / metabolism
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Rats
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Structure-Activity Relationship
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Hepatocyte Nuclear Factor 4
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Peroxisome Proliferators
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Phosphoproteins
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Acetyl-CoA C-Acetyltransferase