Abstract
Positron emission tomography (PET) herpes simplex virus thymidine kinase (HSV-tk) gene reporter probes 9-[(3-[(18)F]fluoro-1-hydroxy-2-propoxy)methyl]guanine ([(18)F]FHPG) and 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl)guanine ([(18)F]FHBG) were prepared by nucleophilic substitution of the appropriate tosylated precursors with [(18)F]KF/Kryptofix 2.2.2 followed by a quick deprotection reaction and purification with a simplified dual Silica Sep-Pak solid-phase extraction (SPE) method in 15-30% radiochemical yield.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Fluorine Radioisotopes
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Ganciclovir / analogs & derivatives
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Genes, Reporter*
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Guanine / analogs & derivatives
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Guanine / chemical synthesis*
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Isotope Labeling / methods
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Simplexvirus / enzymology
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Simplexvirus / genetics*
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Thymidine Kinase / genetics*
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Thymidine Kinase / metabolism*
Substances
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9-(3-fluoro-1-hydroxy-2-propoxy)methylguanine
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9-(4-fluoro-3-hydroxymethylbutyl)guanine
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Fluorine Radioisotopes
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Guanine
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Thymidine Kinase
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Ganciclovir