Evaluation of the expression of collagen type I in porous calcium phosphate ceramics implanted in an extra-osseous site

Biomaterials. 2004 Feb;25(4):659-67. doi: 10.1016/s0142-9612(03)00577-5.

Abstract

The aim of the present study is to demonstrate the newly formed tissue in calcium phosphate (Ca/P) ceramics after extra-osseous implantation by histological and immunohistochemistry (IHC) methods. Synthesis porous Ca/P ceramics without adding any growth factor and living cell were implanted in the dorsal muscle of dogs for 1 and 2 months. Undecalcified and decalcified sections were stained by hematoxylin and eosin (H&E), and IHC, respectively. The histological results showed the beginning of osteogenesis and angiogenesis after being implanted for 1 month and the obvious new bone formation after being implanted for 2 months. IHC were conducted via the avidin-biotin peroxidase complex (ABC) method and the primary antibody was collagen type I. IHC results indicated that collagen type I was expressed within osteoblast-like cells and newly formed bone-like tissue in Ca/P ceramics after 1 month, and in the mineralized matrix of newly formed bone and osteoblasts, some osteocytes and some lacunae after 2 months. No cartilage and chondrocytes were observed in the histological and IHC-stained sections. Evidence of intramembranous osteogenesis was confirmed.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Substitutes / chemistry*
  • Bone Substitutes / metabolism*
  • Calcium Phosphates / chemistry*
  • Ceramics / chemistry
  • Collagen Type I / metabolism*
  • Dogs
  • Materials Testing
  • Muscle, Skeletal / cytology*
  • Muscle, Skeletal / physiology*
  • Muscle, Skeletal / surgery
  • Neovascularization, Physiologic / physiology
  • Osseointegration / physiology
  • Osteogenesis / physiology*
  • Porosity
  • Prostheses and Implants*

Substances

  • Bone Substitutes
  • Calcium Phosphates
  • Collagen Type I
  • calcium phosphate