Abstract
A poly(thionine) modified screen-printed carbon electrode has been prepared by an electrooxidative polymerization of thionine in neutral phosphate buffer. The modified electrodes are found to give stable and reproducible electrocatlytic responses to NADH and exhibit good stability. Several techniques, including cyclic voltammetry, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM), have been employed to characterize the poly(thionine) film. Further, the modified screen-printed carbon electrode was found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 5-100 microM.
Publication types
-
Comparative Study
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
-
Validation Study
MeSH terms
-
Biosensing Techniques / instrumentation*
-
Biosensing Techniques / methods
-
Carbon / chemistry
-
Coated Materials, Biocompatible / chemical synthesis*
-
Coated Materials, Biocompatible / chemistry
-
Electrochemistry / instrumentation*
-
Electrochemistry / methods
-
Electrodes
-
Equipment Design
-
Equipment Failure Analysis
-
Flow Injection Analysis / instrumentation*
-
Flow Injection Analysis / methods
-
NAD / analysis*
-
NAD / chemistry*
-
Phenothiazines / chemical synthesis
-
Phenothiazines / chemistry*
-
Polymers / chemical synthesis
-
Reproducibility of Results
-
Sensitivity and Specificity
-
Surface Properties
Substances
-
Coated Materials, Biocompatible
-
Phenothiazines
-
Polymers
-
NAD
-
Carbon
-
thionine