Elimination by necrosis, not apoptosis, of embryonic extraocular muscles in the muscular dysgenesis mutant of the mouse

Cell Tissue Res. 2004 Feb;315(2):243-7. doi: 10.1007/s00441-003-0831-0. Epub 2003 Nov 14.

Abstract

Muscular dysgenesis (mdg) in the mouse is a loss-of-function mutation of the skeletal muscle isoform of the voltage-sensor Ca2+ channel of skeletal muscle (DHP receptor alpha1 subunit, Cchl1a3, Chr1), which is essential for excitation-contraction coupling. Affected individuals (genotype mdg/mdg, phenotype MDG) are unable to breathe and die perinatally. We introduce here extraocular muscles in the study of MDG myopathy and show that, despite their developmental origin from head placodes, they are affected like trunk and limb muscles. MDG myotubes in situ are eliminated by necrosis, not apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Calcium Channels / genetics*
  • Embryo, Mammalian / physiopathology
  • Mice
  • Mice, Mutant Strains
  • Microscopy, Electron, Scanning Transmission
  • Muscle Contraction / physiology
  • Muscle Fibers, Skeletal / cytology*
  • Muscle, Skeletal / embryology
  • Muscle, Skeletal / physiopathology*
  • Muscular Diseases / physiopathology*
  • Mutation / genetics
  • Necrosis

Substances

  • Calcium Channels