Single-cell analysis of lambda immunity regulation

Kristoffer Baek; Sine Svenningsen; Harvey Eisen; Kim Sneppen; Stanley Brown

doi:10.1016/j.jmb.2003.09.037

Single-cell analysis of lambda immunity regulation

J Mol Biol. 2003 Nov 28;334(3):363-72. doi: 10.1016/j.jmb.2003.09.037.

Authors

Kristoffer Baek¹, Sine Svenningsen, Harvey Eisen, Kim Sneppen, Stanley Brown

Affiliation

¹ Department of Molecular Cell Biology, University of Copenhagen, Øster Farimagsgade 2A, DK-1353, K, Copenhagen, Denmark.

PMID: 14623180
DOI: 10.1016/j.jmb.2003.09.037

Abstract

We have examined expression of the lambdacI operon in single cells via a rex Colon, two colons gfp substitution. Although average fluorescence agreed with expectations for expression of lambda-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Artificial Gene Fusion
Bacteriophage lambda / genetics*
Bacteriophage lambda / immunology
Cells, Cultured / radiation effects
Cells, Cultured / ultrastructure
Coliphages
DNA Repair
Fluorescence
Gene Expression Regulation, Bacterial*
Green Fluorescent Proteins
Immunity, Cellular*
Luminescent Proteins / metabolism
Lysogeny
Operon*
Plasmids
Transcription, Genetic
Ultraviolet Rays

Substances

Luminescent Proteins
Green Fluorescent Proteins