Array of M protein gene subtypes in 1064 recent invasive group A streptococcus isolates recovered from the active bacterial core surveillance

Zhongya Li; Varja Sakota; Delois Jackson; Alma Ruth Franklin; Bernard Beall; Active Bacterial Core Surveillance/Emerging Infections Program Network

doi:10.1086/379050

Array of M protein gene subtypes in 1064 recent invasive group A streptococcus isolates recovered from the active bacterial core surveillance

J Infect Dis. 2003 Nov 15;188(10):1587-92. doi: 10.1086/379050. Epub 2003 Nov 10.

Authors

Zhongya Li¹, Varja Sakota, Delois Jackson, Alma Ruth Franklin, Bernard Beall; Active Bacterial Core Surveillance/Emerging Infections Program Network

Affiliation

¹ Division of Bacterial and Mycotic Diseases, Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

PMID: 14624386
DOI: 10.1086/379050

Abstract

Using sequence analysis to detect variation within the hypervariable M protein N terminus, we found 41 emm types encompassing 81 subtypes, among 1064 consecutive invasive group A streptococcus isolates from a recent multistate, population-based surveillance. Seventeen of the 30 emm types represented by multiple isolates displayed multiple subtypes. Most subtypes differed from reference strain emm sequences as a result of single base substitutions or other alterations likely to be stably inherited. The Centers for Disease Control and Prevention database (available at: http://www.cdc.gov/ncidod/biotech/strep/strepblast.htm) currently contains 225 distinct emm types encompassing 450 subtypes. Although this subtyping scheme increases specificity, limited variation within individual types favors introduction of M protein type-specific vaccines.

MeSH terms

Amino Acid Sequence
Antigens, Bacterial*
Bacterial Outer Membrane Proteins / chemistry
Bacterial Outer Membrane Proteins / genetics*
Bacterial Outer Membrane Proteins / isolation & purification
Base Sequence
Carrier Proteins / chemistry
Carrier Proteins / genetics*
Carrier Proteins / isolation & purification
Centers for Disease Control and Prevention, U.S.
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Genetic Variation
Humans
Molecular Sequence Data
Sequence Alignment
Streptococcal Infections / immunology*
Streptococcal Infections / microbiology
Streptococcal Infections / prevention & control
Streptococcal Vaccines / genetics
Streptococcal Vaccines / immunology
Streptococcus / classification
Streptococcus / genetics*
Streptococcus / immunology
Streptococcus / isolation & purification
United States

Substances

Antigens, Bacterial
Bacterial Outer Membrane Proteins
Carrier Proteins
DNA, Bacterial
Streptococcal Vaccines
streptococcal M protein