Molecular characterization and sequence analysis of human astroviruses circulating in Hungary

FEMS Immunol Med Microbiol. 2003 Nov 28;39(2):97-102. doi: 10.1016/S0928-8244(03)00236-0.

Abstract

Human astroviruses (HAstVs) are major pathogens in viral gastroenteritis worldwide. Twenty-five HAstV strains were detected from stool specimens of children hospitalized for acute gastroenteritis in Budapest, Hungary, between 1995 and 1999. Sequence analysis was performed at the 3' end of the capsid gene to determine genotypic diversity of HAstVs circulating in Hungary. Five different genotypes of HAstVs were identified: HAstV-1 was predominant, followed by types 5, 8, 3 and 4. Two different subtypes of HAstV-1 were detected, but only one at a time in the community. This is the first report on the genetic diversity of HAstVs in Hungary and Central/Eastern Europe.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Astroviridae Infections / epidemiology*
  • Astroviridae Infections / virology*
  • Child
  • Feces / virology
  • Genetic Variation*
  • Genotype
  • Humans
  • Hungary / epidemiology
  • Mamastrovirus / classification
  • Mamastrovirus / genetics*
  • Mamastrovirus / isolation & purification*
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Polymorphism, Genetic
  • RNA, Viral / chemistry
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA / methods

Substances

  • RNA, Viral

Associated data

  • GENBANK/AF395733
  • GENBANK/AF395734
  • GENBANK/AF395735
  • GENBANK/AF395736
  • GENBANK/AF395737
  • GENBANK/AF395738