Abstract
In this study we show that P-glycoprotein in multidrug-resistant 2780AD human ovarian carcinoma cells and multidrug resistance-associated protein 1 in multidrug-resistant HT29col human colon carcinoma cells are predominantly located in Lubrol-based detergent-insoluble glycosphingolipid-enriched membrane domains. This localization is independent of caveolae, since 2780AD cells do not express caveolin-1. Although HT29col cells do express caveolin-1, the ATP-binding cassette transporter and caveolin-1 were dissociated on the basis of differential solubility in Triton X-100 and absence of microscopical colocalization. While both the multidrug resistance-associated protein 1 and caveolin-1 are located in Lubrol-based membrane domains, they occupy different regions of these domains.
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
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ATP-Binding Cassette Transporters / chemistry
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ATP-Binding Cassette Transporters / physiology*
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Adenosine Triphosphate / chemistry*
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Caveolae / metabolism
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Cell Line, Tumor
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Cell Membrane / metabolism
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Chemokines, CC / biosynthesis*
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Detergents / pharmacology
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Drug Resistance, Multiple
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Glycosphingolipids / chemistry
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Humans
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Immunoblotting
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Immunohistochemistry
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Octoxynol / pharmacology
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Polyethylene Glycols / pharmacology
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Precipitin Tests
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Protein Structure, Tertiary
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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ATP-Binding Cassette Transporters
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Chemokines, CC
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Detergents
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Glycosphingolipids
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Ccl6 protein, mouse
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Polyethylene Glycols
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lubrol
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Adenosine Triphosphate
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Octoxynol