A fluorescent DNA probe prepared by the direct derivatization of the sugar moiety for hybridization assay

H Nagaoka; H Nohta; M Saito; Y Ohkura

doi:10.1248/cpb.40.2852

A fluorescent DNA probe prepared by the direct derivatization of the sugar moiety for hybridization assay

Chem Pharm Bull (Tokyo). 1992 Oct;40(10):2852-3. doi: 10.1248/cpb.40.2852.

Authors

H Nagaoka¹, H Nohta, M Saito, Y Ohkura

Affiliation

¹ Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.

PMID: 1464117
DOI: 10.1248/cpb.40.2852

Abstract

The preparation of a fluorescent DNA probe based on the derivatization of the terminal hydroxyl group of the sugar moiety of a DNA primer and its applicability to the DNA hybridization assay are described. M13mp8 plasmid primer reacts with 2-(5-chlorocarbonyl-2-oxazolyl)-5,6-methylenedioxybenzofu ran in the presence of sodium azide to form the corresponding fluorescent probe, which can be used for the hybridization assay to the target DNA, M13mp8 plasmid vector. The detection limit of the DNA with the naked eye is 10 ng (approximately 300 fmol)/spot on filters for the hybridization assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carbohydrates
DNA Probes*
Molecular Sequence Data
Nucleic Acid Hybridization

Substances

Carbohydrates
DNA Probes