Abstract
The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-kappaB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+CD25+ regulatory T-cell-mediated suppressive activities.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Carrier Proteins / genetics*
-
Carrier Proteins / metabolism*
-
DNA Primers
-
DNA, Complementary / genetics
-
Dendritic Cells / metabolism
-
Flow Cytometry
-
Gene Components
-
Gene Expression*
-
Glucocorticoid-Induced TNFR-Related Protein
-
Humans
-
Ligands
-
Luciferases
-
Lymphocytes / metabolism
-
Mice
-
Molecular Sequence Data
-
NF-kappa B / metabolism
-
Plasmids / genetics
-
Receptors, Nerve Growth Factor / metabolism*
-
Receptors, Tumor Necrosis Factor / metabolism*
-
Recombinant Proteins
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sequence Analysis, DNA
-
Suppressor Factors, Immunologic / antagonists & inhibitors
-
Tumor Necrosis Factors
Substances
-
Carrier Proteins
-
DNA Primers
-
DNA, Complementary
-
Glucocorticoid-Induced TNFR-Related Protein
-
Ligands
-
NF-kappa B
-
Receptors, Nerve Growth Factor
-
Receptors, Tumor Necrosis Factor
-
Recombinant Proteins
-
Suppressor Factors, Immunologic
-
TNFRSF18 protein, human
-
Tnfrsf18 protein, mouse
-
Tnfsf18 protein, mouse
-
Tumor Necrosis Factors
-
Luciferases