Stress responses were compared in Chinese water deer (Hydropotes inermis) exposed to different handling, management and disease factors by measuring plasma cortisol and adrenal gland weight. Healthy free-ranging deer shot as part of a management programme served as baseline controls; they had lower cortisol levels than either diseased deer or free-ranging deer that were either netted and manually restrained or anaesthetised by dart. Although darting appeared subjectively to be much less stressful to the deer than manual restraint, there was no significant difference in cortisol levels between the two groups. Hand-reared deer that were manually restrained in a similar manner to the free-ranging deer did not react behaviourally any less violently but showed significantly lower plasma cortisol levels. Adrenal glands of deer with various, mostly chronic, disease conditions weighed significantly more than those of healthy deer.