[Localization and role of dendritic cells in rat kidney with renal ischemia/ reperfusion injury]

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2003 Dec;15(12):726-9.
[Article in Chinese]

Abstract

Objective: To investigate the localization and the role of dendritic cells (DCs) in rat kidney with renal ischemia/reperfusion injury, and to explore the effect of anti-P-selectin Lectin-epidermal growth factor (EGF) domain monoclonal antibody (PsL-EGFmAb) on DCs.

Methods: (1)Rat model of renal ischemia/reperfusion was established and rats were divided into treated group with PsL-EGFmAb (n=20) and untreated group (n=20), which included four sub-groups respectively according to reperfusion time (1-hour,3-hour, 6-hour, 24-hour), sham group (n=5) severed as control. CD1a(+)CD80(+)DC was observed with microscopy images method and P-selectin was analysed with immunohistochemistry.

Results: (1)In renal tissues of untreated group, CD1a(+)CD80(+)DC was found in renal tubules, interstitium and vessels, especially in renal tubules and interstitium, but DCs were hardly observed in sham group (P<0.001). The number of CD1a(+)CD80(+)DC in 24-hour group was greater than those in other groups (P<0.01). The number of DCs was positively associated with blood urea nitrogen and serum creatinine (both P<0.05). (2)P-selectin content was increased significantly after ischemia/reperfusion 1 hour. (3)The contents of DC and P-selectin were decreased after treated (P<0.05 and P<0.01) in rat renal tissues.

Conclusion: DCs play an important role in immune pathogenesis after renal ischemia/reperfusion injury, which is related to renal immigration of DCs mediated by P-selectin. PsL-EGFmAb may inhibit local DCs immigration and accumulation in kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD1 / analysis
  • B7-1 Antigen / analysis
  • Dendritic Cells / physiology*
  • Ischemia / immunology*
  • Kidney / blood supply*
  • Kidney / pathology
  • Kidney / physiopathology
  • Male
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / immunology*

Substances

  • Antigens, CD1
  • B7-1 Antigen
  • CD1a antigen