Abstract
Cohesins hold sister chromatids together from DNA replication until they are segregated. Although cohesins Smc1, Smc3, and Scc1/Rad21 are involved in chromatid cohesion and other cellular processes, little is known about the other mitotic cohesin subunit, Scc3/STAG. Here we describe STAG/Scc3, which may act as a transcriptional co-activator. STAG2 is able to enhance the activity of the tumor necrosis factor alpha, the CD69, and the human immunodeficiency virus long terminal repeat promoters in a NF-kappaB-dependent manner. In addition, STAG2 interacts with the viral transactivator Tat and enhances the Tat-mediated activation of the human immunodeficiency virus long terminal repeat promoter. Moreover, STAG2 co-activates a multimeric NF-kappaB reporter construct and enhances the activity of the transactivation domain of p65/RelA in a Gal4 system. This function is dependent on one of the LXXLL co-activation motives present in this cohesin and is substantiated by the interaction of STAG2 with the p65 subunit of NF-kappaB. These results describe a novel activity for cohesins, suggesting a role for STAG/Scc3 in transcriptional regulation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Motifs
-
Antigens, CD / metabolism
-
Antigens, Differentiation, T-Lymphocyte / metabolism
-
Cell Cycle Proteins
-
Cell Line
-
Chromosomal Proteins, Non-Histone
-
Cohesins
-
DNA / chemistry
-
DNA-Binding Proteins
-
Fungal Proteins
-
HIV Long Terminal Repeat
-
Humans
-
Jurkat Cells
-
K562 Cells
-
Lectins, C-Type
-
NF-kappa B / metabolism
-
Nuclear Proteins / chemistry*
-
Nuclear Proteins / genetics*
-
Nuclear Proteins / metabolism
-
Plasmids / metabolism
-
Precipitin Tests
-
Promoter Regions, Genetic
-
Protein Binding
-
Protein Structure, Tertiary
-
Saccharomyces cerevisiae Proteins / metabolism
-
Time Factors
-
Trans-Activators / chemistry*
-
Trans-Activators / genetics*
-
Transcription Factors / metabolism
-
Transcription, Genetic
-
Transcriptional Activation*
-
Tumor Necrosis Factor-alpha / metabolism
Substances
-
Antigens, CD
-
Antigens, Differentiation, T-Lymphocyte
-
CD69 antigen
-
Cell Cycle Proteins
-
Chromosomal Proteins, Non-Histone
-
DNA-Binding Proteins
-
Fungal Proteins
-
GAL4 protein, S cerevisiae
-
Lectins, C-Type
-
NF-kappa B
-
Nuclear Proteins
-
Saccharomyces cerevisiae Proteins
-
Stag2 protein, mouse
-
Trans-Activators
-
Transcription Factors
-
Tumor Necrosis Factor-alpha
-
DNA