Abstract
Interactions of nuclear extract and nuclear matrix proteins from rat hepatocytes with the hormone response element of the alpha2-macroglobulin gene were studied. By Western and South-Western blot analysis we have shown the presence of C/EBPbeta in the examined nuclear fractions as well as its increased binding affinity to the examined gene sequence during the acute-phase response. The results suggest that both nuclear protein fractions could participate in the transcriptional regulation of the alpha2-macroglobulin gene in the rat hepatocytes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute-Phase Reaction / chemically induced
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Acute-Phase Reaction / immunology
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Acute-Phase Reaction / metabolism*
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Animals
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CCAAT-Enhancer-Binding Proteins / immunology
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CCAAT-Enhancer-Binding Proteins / metabolism*
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Cell Nucleus / immunology
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Cell Nucleus / metabolism*
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Cells, Cultured
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Hepatocytes / immunology
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Hepatocytes / metabolism*
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Male
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Nuclear Matrix / immunology
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Nuclear Matrix / metabolism
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Rats
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Rats, Wistar
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Transcription Factor CHOP
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Transcription Factors / immunology
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Transcription Factors / metabolism*
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Turpentine
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alpha-Macroglobulins / immunology
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alpha-Macroglobulins / metabolism*
Substances
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CCAAT-Enhancer-Binding Proteins
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Ddit3 protein, rat
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Transcription Factors
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alpha-Macroglobulins
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Transcription Factor CHOP
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Turpentine