Abstract
Transgenic (Tg) L2 mice expressing high levels of the lambda2 (315) L chain contain only B cell populations involved in the first line of defense, i.e., B-1 and marginal zone (MZ) B cells. The strongly oligoclonal IgH chain repertoire of Tg B-1a cells in such mice was attributed to strong positive selection by autoantigens. In this study, we show that the MZ B cells of L2 mice correspond very closely to MZ B cells of normal mice, as revealed by surface marker expression and gene expression profiling. We demonstrate that the IgH chain repertoire of these Tg MZ B cells is extremely heterogeneous. This is in sharp contrast to the oligoclonality found in B-1a cells of the same mice, which was attributed to strong positive selection mediated by autoantigens. Therefore, the strong positive selection of the IgH chain repertoire in L2 mice is B-1a specific. Thus, our data demonstrate that despite common functional properties, MZ B and B-1a cells exhibit striking differences in their selection and/or maintenance requirements.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocyte Subsets / cytology*
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B-Lymphocyte Subsets / immunology*
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B-Lymphocyte Subsets / metabolism
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B-Lymphocyte Subsets / pathology
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology
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Bone Marrow Cells / metabolism
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Bone Marrow Cells / pathology
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Gene Expression Profiling
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Gene Rearrangement, B-Lymphocyte, Heavy Chain
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Immunoglobulin Heavy Chains / biosynthesis
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Light Chains / biosynthesis
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Immunoglobulin Light Chains / genetics
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Immunoglobulin mu-Chains / biosynthesis
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Immunoglobulin mu-Chains / genetics
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Mice
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Mice, Inbred BALB C
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Mice, Transgenic
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Molecular Sequence Data
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Peritoneum / immunology
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Peritoneum / metabolism
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Peritoneum / pathology
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Receptors, Complement 3d / biosynthesis
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Receptors, IgE / biosynthesis
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Spleen / immunology
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Spleen / metabolism
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Spleen / pathology
Substances
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin mu-Chains
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Receptors, Complement 3d
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Receptors, IgE