Comparison of the sequences of the internal transcribed spacer regions and PbGP43 genes of Paracoccidioides brasiliensis from patients and armadillos (Dasypus novemcinctus)

Flavia Hebeler-Barbosa; Flavia V Morais; Mario R Montenegro; Eiko E Kuramae; Beatriz Montes; Juan G McEwen; Eduardo Bagagli; Rosana Puccia

doi:10.1128/JCM.41.12.5735-5737.2003

Comparison of the sequences of the internal transcribed spacer regions and PbGP43 genes of Paracoccidioides brasiliensis from patients and armadillos (Dasypus novemcinctus)

J Clin Microbiol. 2003 Dec;41(12):5735-7. doi: 10.1128/JCM.41.12.5735-5737.2003.

Authors

Flavia Hebeler-Barbosa¹, Flavia V Morais, Mario R Montenegro, Eiko E Kuramae, Beatriz Montes, Juan G McEwen, Eduardo Bagagli, Rosana Puccia

Affiliation

¹ Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, SP, Brazil.

Abstract

Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates differed by one or three sites among five total substitution sites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Fungal / genetics*
DNA, Intergenic / genetics*
Fungal Proteins / genetics*
Glycoproteins / genetics*
Humans
Mammals
Molecular Sequence Data
Paracoccidioides / classification
Paracoccidioides / genetics*
Paracoccidioides / isolation & purification
Phylogeny
Polymorphism, Single Nucleotide

Substances

43 kDa protein, Paracoccidioides
Antigens, Fungal
DNA, Intergenic
Fungal Proteins
Glycoproteins

Associated data

GENBANK/AY374336
GENBANK/AY374339