Molecular detection and identification of agents of eumycetoma: detailed report of two cases

Abdalla O A Ahmed; Nicole Desplaces; Philippe Leonard; Fred Goldstein; Sybren De Hoog; Henri Verbrugh; Alex van Belkum

doi:10.1128/JCM.41.12.5813-5816.2003

Molecular detection and identification of agents of eumycetoma: detailed report of two cases

J Clin Microbiol. 2003 Dec;41(12):5813-6. doi: 10.1128/JCM.41.12.5813-5816.2003.

Authors

Abdalla O A Ahmed¹, Nicole Desplaces, Philippe Leonard, Fred Goldstein, Sybren De Hoog, Henri Verbrugh, Alex van Belkum

Affiliation

¹ Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

Abstract

We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.

Publication types

Case Reports

MeSH terms

Adult
Antifungal Agents / therapeutic use
Ascomycota / genetics*
Ascomycota / isolation & purification*
DNA, Fungal / genetics
DNA, Fungal / isolation & purification
Female
Humans
Itraconazole / therapeutic use
Magnetic Resonance Imaging
Male
Middle Aged
Mycoses / diagnosis*
Mycoses / pathology
Mycoses / therapy
Polymerase Chain Reaction / methods

Substances

Antifungal Agents
DNA, Fungal
Itraconazole