Abstract
Representational difference analysis, a cDNA subtraction approach, was used to identify genes that are expressed in acute leukemia but not in normal hematological tissues. We isolated a cDNA fragment from a cell line derived from a B cell acute lymphoblastic leukemia bearing two Philadelphia chromosomes. The cDNA derives from an orphan gene that was named BLACE. BLACE is located in region 7q36 and encodes a major 5.3-kb transcript and several alternatively spliced minor transcripts. Significant expression of BLACE was detected by RT-PCR and quantitative RT-PCR in bone marrow samples from B cell acute lymphoblastic leukemia patients. BLACE was not or was scarcely expressed in other types of hematological malignancies, in normal tissues, and in solid tumors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Blotting, Northern
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Burkitt Lymphoma / genetics*
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Burkitt Lymphoma / pathology
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Cell Line, Tumor
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Chromosome Mapping
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Chromosomes, Human, Pair 7 / genetics
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Exons
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic*
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Genes / genetics
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Male
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Middle Aged
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Molecular Sequence Data
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Neoplasm Proteins / genetics*
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RNA, Long Noncoding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Radiation Hybrid Mapping
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Sequence Analysis, DNA
Substances
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BLACE lncRNA, human
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DNA, Complementary
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Neoplasm Proteins
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RNA, Long Noncoding
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RNA, Messenger