Molecular mechanisms for viral mimicry of a human cytokine: activation of gp130 by HHV-8 interleukin-6

J Mol Biol. 2004 Jan 9;335(2):641-54. doi: 10.1016/j.jmb.2003.10.070.

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV, or HHV-8) encodes a pathogenic viral homologue of human interleukin-6 (IL-6). In contrast to human IL-6 (hIL-6), viral IL-6 (vIL-6) binds directly to, and activates, the shared human cytokine signaling receptor gp130 without the requirement for pre-complexation to a specific alpha-receptor. Here, we dissect the biochemical and functional basis of vIL-6 mimicry of hIL-6. We find that, in addition to the "alpha-receptor-independent" tetrameric vIL-6/gp130 complex, the viral cytokine can engage the human alpha-receptor (IL-6Ralpha) to form a hexameric vIL-6/IL-6Ralpha/gp130 complex with enhanced signaling potency. In contrast to the assembly sequence of the hIL-6 hexamer, the preformed vIL-6/gp130 tetramer can be decorated with IL-6Ralpha, post facto, in a "vIL-6-dependent" fashion. A detailed comparison of the viral and human cytokine/gp130 interfaces indicates that vIL-6 has evolved a unique molecular strategy to interact with gp130, as revealed by an almost entirely divergent structural makeup of its receptor binding sites. Viral IL-6 appears to utilize an elegant combination of both convergent, and unexpectedly divergent, molecular strategies to oligomerize gp130 and activate similar downstream signaling cascades as its human counterpart.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD / chemistry
  • Antigens, CD / metabolism*
  • Cytokine Receptor gp130
  • Herpesvirus 8, Human / metabolism*
  • Humans
  • Interleukin-6 / chemistry
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / metabolism*
  • Molecular Mimicry*
  • Molecular Sequence Data
  • Protein Binding
  • Receptors, Interleukin-6 / physiology*
  • Sarcoma, Kaposi
  • Sequence Homology, Amino Acid
  • Signal Transduction*
  • Structure-Activity Relationship
  • Thermodynamics
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • Antigens, CD
  • IL6ST protein, human
  • Interleukin-6
  • Membrane Glycoproteins
  • Receptors, Interleukin-6
  • Viral Proteins
  • interleukin-6 receptor alpha
  • Cytokine Receptor gp130