Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from eastern Slovakia

Ann Agric Environ Med. 2003;10(2):269-71.

Abstract

Ixodes ricinus ticks (20 males, 20 females and 20 nymphs) collected in Kosice, Slovakia were examined for the presence of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato (s.l.) by PCR. 38.3 % of the tested ticks carried single infection of B. burgdorferi s.l. and 8.3 % were infected with A. phagocytophilum. Double infection of both pathogens was detected in 5 % of tested ticks. These results indicate that both B. burgdorferi s.l. and A. phagocytophilum co-circulate in the enzootic sites of Eastern Slovakia and may cause co-infection in humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaplasma phagocytophilum / genetics*
  • Anaplasma phagocytophilum / isolation & purification
  • Animals
  • Borrelia burgdorferi Group / genetics*
  • Borrelia burgdorferi Group / isolation & purification
  • DNA Primers
  • DNA, Bacterial / analysis
  • Disease Reservoirs
  • Ehrlichiosis / epidemiology
  • Ehrlichiosis / microbiology
  • Female
  • Humans
  • Ixodes / microbiology*
  • Lyme Disease / epidemiology
  • Lyme Disease / microbiology*
  • Male
  • Polymerase Chain Reaction
  • Prevalence
  • Slovakia / epidemiology

Substances

  • DNA Primers
  • DNA, Bacterial