Abstract
We isolated and sequenced both genomic DNA and cDNA clones, which encoded a putative GT1-like protein with 385 amino acids, from cultivated rice (Oryza sativa ssp. indica). This protein shows significant amino acid sequence similarities with trihelix DNA-binding GT-1a/B2F and GT-1 factors that were identified in dicot plants. Northern blotting analysis indicated that the transcript of the rice GT-1 factor in seedling was up-regulated by the rice blast fungus Magnaporthe grisea, down-regulated by various continuous light conditions and expressed rhythmically in light/dark cycles. This GT1-like factor gene was therefore designated as rml1 (rice gene regulated by M. grisea and light). The putative RML1 protein, encoded by this single copy gene, is thus identified as a new member of the plant-specific GT family of transcription factors in rice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Circadian Rhythm
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA, Plant / chemistry
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DNA, Plant / genetics
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DNA-Binding Proteins / genetics*
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Down-Regulation
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Gene Expression Regulation, Plant / radiation effects
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Light
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Magnaporthe / growth & development*
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Molecular Sequence Data
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Oryza / genetics*
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Oryza / microbiology
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Oryza / radiation effects
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Plant Leaves / genetics
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Plant Leaves / microbiology
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Plant Leaves / radiation effects
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Plant Proteins / genetics*
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Transcription, Genetic / radiation effects
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Up-Regulation
Substances
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DNA, Complementary
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DNA, Plant
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DNA-Binding Proteins
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Plant Proteins
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RNA, Plant
Associated data
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GENBANK/AJ535489
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GENBANK/AJ544589