Abstract
TGF beta-inducible immediate early gene, Tieg, belongs to the superfamily of Sp1-like transcription factors containing three C(2)H(2)-zinc finger DNA binding motifs close to the C-terminus. So far, Tieg1 and Tieg2 have been identified in human and mouse. We identified Tieg3, a new member of the Tieg protein family by screening a mouse cDNA library. Tieg3 has almost all the known features of the Tieg protein family: it shares a highly conserved C(2)H(2) zinc finger DNA binding domain and is 96% identical to Tieg2 and 86% to Tieg1, respectively. In addition, the three repression domains at the N-terminus, R1, R2 and R3 are conserved in all the Tiegs. Similar to Tieg1 and Tieg2, Tieg3 mRNA is up-regulated in response to TGF beta 1 treatment and can perform the Sp1 sites mediated repression of transcription. A 4 kilobase (kb) long transcript of mouse Tieg3 can be detected using Northern-blot analysis. The gene of mouse Tieg3 contains four exons. Due to the amino acid sequence similarity, mouse Tieg2 is regarded as an orthologue of human Tieg2. However, the mouse Tieg3 gene is localized in a conserved segment on mouse chromosome 12 corresponding to human Tieg2 on chromosome 2 with the same gene order. An interesting explanation for this apparent contradiction might be a homologous recombination leading to loci exchange between the mouse Tieg3 and Tieg2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Apoptosis Regulatory Proteins
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Base Sequence
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Cell Cycle Proteins / genetics
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 2 / genetics
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Chromosomes, Mammalian / genetics
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Cloning, Molecular
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DNA / genetics
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DNA / metabolism
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DNA Restriction Enzymes / metabolism
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Early Growth Response Transcription Factors
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Evolution, Molecular*
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Exons
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Gene Expression Regulation / drug effects
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Genes / genetics
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Humans
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Introns
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Kruppel-Like Transcription Factors
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Mice
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Molecular Sequence Data
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Multigene Family / genetics
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Oligodendroglia / cytology
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Oligodendroglia / drug effects
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Oligodendroglia / metabolism
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Poly A / genetics
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Polymorphism, Restriction Fragment Length
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Repressor Proteins / genetics
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Synteny
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transforming Growth Factor beta / pharmacology
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Transforming Growth Factor beta1
Substances
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Apoptosis Regulatory Proteins
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Cell Cycle Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Early Growth Response Transcription Factors
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KLF10 protein, human
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KLF11 protein, human
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KLF11 protein, mouse
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Kruppel-Like Transcription Factors
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RNA, Messenger
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Repressor Proteins
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TGFB1 protein, human
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Tgfb1 protein, mouse
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Tieg1 protein, mouse
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Transcription Factors
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Transforming Growth Factor beta
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Transforming Growth Factor beta1
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Poly A
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DNA
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DNA Restriction Enzymes