Abstract
M1 mouse leukemia cells differentiate to macrophages/monocytes by the stimulation of interleukin-6 (IL-6)/leukemia inhibitory factor (LIF). To identify new LIF-induced genes, we have performed representational difference analysis using M1 cells and cloned mouse interleukin-3 (IL-3) receptor beta subunit gene. The mRNA expression of both IL-3 receptor (IL-3R) alpha and beta subunits is upregulated after 1 h stimulation of LIF and remains to be elevated along the differentiation of M1 cells. This induction is almost completely suppressed in M1 cells expressing a dominant negative form of Stat3. Furthermore, we show that IL-3-induced Stat5 phosphorylation increases in LIF-stimulated M1 cells. These results suggest that Stat3 may play a role in the differentiation of myeloid cells by regulating IL-3R expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution / genetics
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Animals
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Blotting, Northern
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Bone Marrow Cells / chemistry
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Cell Differentiation / physiology
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Cell Line
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Cell Line, Tumor
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology*
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Gene Expression / drug effects
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Gene Expression Regulation, Leukemic / drug effects*
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Interleukin-3 / pharmacology
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Interleukin-6 / pharmacology*
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Interleukin-6 / physiology
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Leukemia Inhibitory Factor
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Leukemia, Myeloid, Acute / genetics
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Leukemia, Myeloid, Acute / pathology
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Mice
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Milk Proteins*
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Phosphorylation / drug effects
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Point Mutation / genetics
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Protein Subunits / genetics
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Receptors, Interleukin-3 / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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STAT3 Transcription Factor
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STAT5 Transcription Factor
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Signal Transduction / physiology
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Trans-Activators / physiology*
Substances
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DNA-Binding Proteins
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Interleukin-3
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Interleukin-6
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Leukemia Inhibitory Factor
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Lif protein, mouse
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Milk Proteins
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Protein Subunits
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
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Receptors, Interleukin-3
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STAT3 Transcription Factor
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STAT5 Transcription Factor
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Stat3 protein, mouse
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Trans-Activators