[Correlation between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability in rats]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003 Dec;32(6):465-9. doi: 10.3785/j.issn.1008-9292.2003.06.002.
[Article in Chinese]

Abstract

Objective: To investigate the relationship between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability of rats.

Methods: Using a novel-object recognition model and Morris water maze,the novel-object recognition ability and spatial memory of SD rats were ranked, and grouped as the high (top 20 %) and the low (bottom 20%), respectively. NR1 subunit protein levels in hippocampus were measured by quantitative immunoblotting with NR1 subunit specific antibody.

Result: The level of NR1 subunit protein in hippocampus in the high novel-object recognition ability group was 60% (P<0.01), higher than that in the low one, and in the high spatial memory group it was 45.4 % (P<0.05), higher than that in the low one, respectively.

Conclusion: The basic expression level of NR1 subunit protein in hippocampus is related to novel-object recognition ability and spatial memory of rats.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hippocampus / chemistry*
  • Hippocampus / physiology
  • Immunoblotting
  • Learning*
  • Male
  • Maze Learning
  • Rats
  • Rats, Sprague-Dawley
  • Reaction Time
  • Receptors, N-Methyl-D-Aspartate / analysis*
  • Receptors, N-Methyl-D-Aspartate / physiology

Substances

  • NR1 NMDA receptor
  • Receptors, N-Methyl-D-Aspartate