Assays and affinity purification of biotinylated and nonbiotinylated forms of double-tagged core RNA polymerase II from Saccharomyces cerevisiae

Methods Enzymol. 2003:370:138-55. doi: 10.1016/S0076-6879(03)70012-3.
No abstract available

MeSH terms

  • Biochemistry / methods*
  • Biotinylation
  • Carbon-Nitrogen Ligases / chemistry
  • DNA / chemistry
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / chemistry
  • Glutathione Transferase / metabolism
  • Histidine / chemistry
  • Nickel / chemistry
  • Protein Binding
  • RNA Polymerase II / chemistry*
  • Repressor Proteins / chemistry
  • Saccharomyces cerevisiae / enzymology*
  • Sepharose / chemistry
  • Time Factors
  • Transcription Factors / chemistry

Substances

  • Escherichia coli Proteins
  • Repressor Proteins
  • Transcription Factors
  • Histidine
  • Nickel
  • DNA
  • Sepharose
  • Glutathione Transferase
  • RNA Polymerase II
  • Carbon-Nitrogen Ligases
  • birA protein, E coli