Formation of tannin-albumin nano-particles at neutral pH as measured by light scattering techniques

Huan-Chang Lin; Po-Chung Chen; Tzong-Jih Cheng; Richie L C Chen

doi:10.1016/j.ab.2003.10.013

Formation of tannin-albumin nano-particles at neutral pH as measured by light scattering techniques

Anal Biochem. 2004 Feb 1;325(1):117-20. doi: 10.1016/j.ab.2003.10.013.

Authors

Huan-Chang Lin¹, Po-Chung Chen, Tzong-Jih Cheng, Richie L C Chen

Affiliation

¹ Department of Bio-Industrial Mechatronics Engineering, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, Taiwan ROC.

PMID: 14715291
DOI: 10.1016/j.ab.2003.10.013

Abstract

Aggregation phenomena of tannin with bovine serum albumin were investigated by light scattering techniques including photon correlation spectroscopy and Rayleigh scattering. Tannin and albumin formed particles with diameters less than 1 microm at neutral pH. As revealed by this study, light scattering methods are useful in investigating aggregation phenomena of biomolecules and in directly quantifying tannin content.

MeSH terms

Animals
Cattle
Hydrogen-Ion Concentration
Nanotechnology
Particle Size
Scattering, Radiation
Serum Albumin, Bovine / analysis*
Tannins / analysis*

Substances

Tannins
Serum Albumin, Bovine