Mutations including IS6110 insertion in the gene encoding the MPB64 protein of Capilia TB-negative Mycobacterium tuberculosis isolates

J Clin Microbiol. 2004 Jan;42(1):390-2. doi: 10.1128/JCM.42.1.390-392.2004.

Abstract

A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, Bacterial / analysis
  • Antigens, Bacterial / genetics*
  • Antigens, Bacterial / immunology
  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / immunology
  • Chromatography
  • DNA Transposable Elements
  • Mutagenesis, Insertional
  • Mutation*
  • Mycobacterium tuberculosis / genetics*

Substances

  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Bacterial Proteins
  • DNA Transposable Elements
  • MPB64 protein, Mycobacterium