Dendrite development regulated by CREST, a calcium-regulated transcriptional activator

Science. 2004 Jan 9;303(5655):197-202. doi: 10.1126/science.1089845.

Abstract

The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain / cytology
  • Brain / embryology
  • Brain / growth & development
  • Brain / metabolism
  • CREB-Binding Protein
  • Calcium / metabolism*
  • Calcium Channels / metabolism
  • Cell Line
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Cerebral Cortex / embryology
  • Cerebral Cortex / metabolism
  • Cloning, Molecular
  • Dendrites / physiology*
  • Dendrites / ultrastructure
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Gene Targeting
  • Humans
  • In Situ Hybridization
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Mutation
  • Nervous System / embryology
  • Nervous System / growth & development
  • Nervous System / metabolism
  • Neurons / physiology*
  • Neurons / ultrastructure
  • Nuclear Proteins / metabolism
  • Protein Structure, Tertiary
  • Rats
  • Recombinant Fusion Proteins / metabolism
  • Trans-Activators / chemistry
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription, Genetic*
  • Transcriptional Activation*
  • Transfection

Substances

  • Calcium Channels
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Ss18l1 protein, rat
  • Trans-Activators
  • CREB-Binding Protein
  • CREBBP protein, human
  • Crebbp protein, mouse
  • Crebbp protein, rat
  • Calcium