Abstract
A recombinant peptide fragment of vitronectin (rVN143), that includes the Arg-Gly-Asp (RGD) cell recognition site, was expressed in Escherichia coli using a prokaryotic expression system. The addition of recombinant rVN143 peptide enhances cell adhesion and proliferation similar (approximately 70%) to those of native VN.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Biomimetic Materials / metabolism*
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Cell Adhesion / drug effects
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Cell Adhesion / physiology
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Cell Division / drug effects
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Cell Division / physiology
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Cloning, Molecular
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Dose-Response Relationship, Drug
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Fibroblasts / cytology
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Fibroblasts / drug effects*
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Fibroblasts / physiology*
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Gene Expression Regulation, Bacterial / physiology
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Gingiva / metabolism
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Humans
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Peptide Fragments / biosynthesis
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Peptide Fragments / chemistry
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Protein Binding
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Protein Engineering / methods*
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Structure-Activity Relationship
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Vitronectin / biosynthesis*
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Vitronectin / classification
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Vitronectin / pharmacology*
Substances
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Peptide Fragments
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Vitronectin