Immunosuppressive antimetabolites inhibit induction of contact hypersensitivity while lymphoablative drugs also prevent its expression

Eur J Dermatol. 2003 Nov-Dec;13(6):540-7.

Abstract

Contact hypersensitivity is one of the most common skin diseases and its pharmacological control is an important clinical issue. We investigated the control of contact hypersensitivity by immunosuppressive drugs administered during sensitization or challenge. Mycophenolate mofetil, methotrexate and 5-fluorouracil completely inhibited contact hypersensitivity when administered during sensitization whereas they did not decrease inflammatory reaction when administered during challenge. Conversely, mitoxantrone, and cyclophosphamide, given as a single injection at the time of sensitization or challenge, completely inhibited the reaction, a property associated with T and B cell depletion. The data indicate that antimetabolites which are cell cycle dependent inhibit clonal expansion and subsequent differentiation of cytotoxic CD8+ T cells. Their lack of effect at the time of challenge indicates that T cell proliferation is not required for the expression of effector or regulatory T cell activation. Conversely lymphoablative drugs can inactivate or destroy differentiated cytotoxic T cells with rapid kinetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimetabolites / pharmacology*
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • CD8-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / immunology
  • Cell Movement
  • Cells, Cultured
  • Cyclophosphamide / pharmacology
  • Dermatitis, Allergic Contact / immunology*
  • Dermatitis, Allergic Contact / prevention & control
  • Dinitrofluorobenzene
  • Female
  • Fluorouracil / pharmacology
  • Immunosuppressive Agents / pharmacology*
  • Langerhans Cells / immunology
  • Lymphocytes / drug effects*
  • Methotrexate / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Mitoxantrone / pharmacology
  • Mycophenolic Acid / analogs & derivatives*
  • Mycophenolic Acid / pharmacology

Substances

  • Antimetabolites
  • Antineoplastic Agents
  • Immunosuppressive Agents
  • Cyclophosphamide
  • Mitoxantrone
  • Dinitrofluorobenzene
  • Mycophenolic Acid
  • Fluorouracil
  • Methotrexate