An inorganic iron complex that inhibits wild-type and an isoniazid-resistant mutant 2-trans-enoyl-ACP (CoA) reductase from Mycobacterium tuberculosis

Jaim S Oliveira; Eduardo H S Sousa; Luiz A Basso; Moisés Palaci; Reynaldo Dietze; Diógenes S Santos; Icaro S Moreira

doi:10.1039/b313592f

An inorganic iron complex that inhibits wild-type and an isoniazid-resistant mutant 2-trans-enoyl-ACP (CoA) reductase from Mycobacterium tuberculosis

Chem Commun (Camb). 2004 Feb 7:(3):312-3. doi: 10.1039/b313592f. Epub 2004 Jan 7.

Authors

Jaim S Oliveira¹, Eduardo H S Sousa, Luiz A Basso, Moisés Palaci, Reynaldo Dietze, Diógenes S Santos, Icaro S Moreira

Affiliation

¹ Universidade Federal do Rio Grande do Sul, Departamento de Biologia Molecular e Biotecnologia, Av. Bento Gonçalves 9500, Porto Alegre, RS 91501-970, Brazil. [email protected]

PMID: 14740053
DOI: 10.1039/b313592f

Abstract

The in vitro kinetics of inactivation of both wild-type and I21V InhA enzymes by [FeII(CN)5(INH)]3- indicate that this process requires no activation by KatG, and no need for the presence of NADH. This inorganic complex may represent a new class of lead compounds to the development of anti-tubercular agents aiming at inhibition of a validated target.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drug Resistance, Bacterial*
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)
Iron / pharmacology*
Isoniazid / pharmacology*
Molecular Structure
Mutation / genetics*
Mycobacterium tuberculosis / enzymology*
NAD / metabolism
Oxidoreductases / antagonists & inhibitors*
Oxidoreductases / genetics*
Oxidoreductases / metabolism

Substances

NAD
Iron
Oxidoreductases
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)
Isoniazid