Functional genomic analysis of the response of HIV-1-infected lymphatic tissue to antiretroviral therapy

J Infect Dis. 2004 Feb 15;189(4):572-82. doi: 10.1086/381396. Epub 2004 Feb 5.

Abstract

Highly active antiretroviral therapy (HAART) curtails human immunodeficiency virus type 1 (HIV-1) replication in lymphatic tissues and partially reverses the pathological damage associated with infection, but the genes that mediate these pathological and reparative processes remain largely unknown. To identify these genes, we used microarrays to profile gene expression in serial lymph node biopsy specimens obtained before and after treatment. We discovered approximately 200 treatment-responsive genes, many of them known mediators and moderators of immune activation and defenses, particularly innate defense genes, which, surprisingly, were expressed at all stages of HIV-1 infection. Most of the rest of the treatment-responsive genes we categorized as mediators of trafficking, reformation of active follicles, and tissue repair. We propose a model in which nearly counterbalanced functions of mediators and moderators of immune activation and defenses account for the slow dynamics of HIV-1 infection before treatment. This model suggests that there could be a role for anti-inflammatory agents, alone or in combination with HAART, in treating HIV-1 infection by tipping this balance to mitigate pathology.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acquired Immunodeficiency Syndrome / drug therapy*
  • Acquired Immunodeficiency Syndrome / genetics*
  • Acquired Immunodeficiency Syndrome / pathology
  • Anti-HIV Agents / therapeutic use*
  • Antiretroviral Therapy, Highly Active
  • Biopsy
  • DNA Primers
  • Genomics
  • HIV Infections / drug therapy
  • HIV Infections / genetics
  • HIV Infections / pathology
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • Lymph Nodes / pathology*
  • Lymphoid Tissue / drug effects
  • Lymphoid Tissue / pathology
  • Lymphoid Tissue / virology
  • Oligonucleotide Array Sequence Analysis
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification

Substances

  • Anti-HIV Agents
  • DNA Primers
  • RNA, Viral