Secondary structure at the beginning of the poly(A) sequence of mouse beta-actin messenger RNA

Biochimie. 1992 Nov;74(11):1031-4. doi: 10.1016/0300-9084(92)90024-9.

Abstract

A portion of the beta-actin mRNA of mammalian cells is believed to lack a poly(A) tail, because of its failure to bind to oligo(dT)-cellulose. S1 mapping and Northern blot analysis of this mRNA shows it to contain a poly(A) sequence of about 60 nucleotides. Only about 20-40 nucleotides are available for interaction with oligo(dT). The rest is masked, presumably by base-pairing with a poly(U) stretch present in the 3' non-coding region of the mRNA. A similar configuration occurs in the bulk of the actin mRNA, which carries a poly(A) tail with sizes ranging from approximately 60 to 200 nucleotides.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics*
  • Animals
  • Blotting, Northern
  • Mice
  • Nucleic Acid Conformation*
  • Oligodeoxyribonucleotides / chemistry
  • Poly A / chemistry*
  • RNA, Messenger / chemistry*
  • Sarcoma 180
  • Tumor Cells, Cultured

Substances

  • Actins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • oligo (dT)
  • Poly A