Abstract
To understand better the organization and linkage of the interleukin genes, IL4 and IL5, we prepared long-range restriction maps of five yeast artificial chromosomes (YACs) containing IL5. We determined that IL4 and IL5 are within 100-170 kb, and that the regions surrounding these genes contain several GC-rich areas. Fluorescence in situ chromosomal analysis demonstrated that three of the five YAC clones contain non-contiguous genomic sequences originating from multiple human chromosomes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Chromosome Mapping
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Chromosomes, Fungal
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Chromosomes, Human, Pair 5*
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DNA, Single-Stranded
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Electrophoresis, Gel, Pulsed-Field
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Genetic Linkage
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Genome, Human
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Genomic Library*
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Humans
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In Situ Hybridization, Fluorescence
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Interleukin-4 / genetics*
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Interleukin-5 / genetics*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Restriction Mapping
Substances
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DNA, Single-Stranded
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Interleukin-5
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Interleukin-4