Generation and characterization of radiation reduced cell hybrids and isolation of probes from the proximal short arm of the human X chromosome

W Berger; A Meindl; B de Leeuw; A de Roos; T J van de Pol; T Meitinger; S D van der Velde-Visser; H Achatz; A Geurts van Kessel; F P Cremers

doi:10.1007/BF00220070

Generation and characterization of radiation reduced cell hybrids and isolation of probes from the proximal short arm of the human X chromosome

Hum Genet. 1992 Nov;90(3):243-6. doi: 10.1007/BF00220070.

Authors

W Berger¹, A Meindl, B de Leeuw, A de Roos, T J van de Pol, T Meitinger, S D van der Velde-Visser, H Achatz, A Geurts van Kessel, F P Cremers, et al.

Affiliation

¹ Department of Human Genetics, University Hospital Nijmegen, The Netherlands.

PMID: 1487237
DOI: 10.1007/BF00220070

Abstract

Employing a modified Goss-Harris irradiation fusion protocol, we have generated a panel of somatic cell hybrids containing various overlapping fragments of the Xcen-Xp11.4 interval. This region of the human X chromosome is known to carry genes for several hereditary eye diseases including retinitis pigmentosa (RP2), congenital stationary night blindness (CSNB-1) and Norrie disease. These hybrid cell lines were employed to isolate 17 new DNA probes by making use of the Alu polymerase chain reaction (PCR) method and subsequent cloning of the PCR products in a plasmid vector. With these probes, we have characterized two previously described microdeletions spanning the Norrie locus; these deletions have enabled us to subdivide the Xp11.4-p11.3 region into three defined intervals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Chromosome Mapping
Cricetinae
Cricetulus
DNA Probes / isolation & purification*
DNA, Single-Stranded
Humans
Hybrid Cells*
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Plasmids
Polymerase Chain Reaction
X Chromosome*

Substances

DNA Probes
DNA, Single-Stranded