A multiparametric study of malignant lymphoma of mucosa associated lymphoid tissue (MALT)

Leuk Lymphoma. 1992 Sep;8(1-2):87-96. doi: 10.3109/10428199209049822.

Abstract

A morphological, immunophenotypic and ultrastructural study, cell cycle estimation, DNA and cytogenetic analysis were performed in ten cases of B-MALT lymphomas. Five had low grade lymphoma and five had high grade. Low and high grade cases showed the same cells but in different percentages: These included centrocyte-like cells with occasional monocytoid cytoplasmic changes, and centroblast-like cells. However, in high grade cases more dysplastic and large cells were present. All cellular types showed an important development of rough endoplasmic reticulum. In all cases a large panel of monoclonal antibodies was employed to study the B-cell immunophenotype. Ki-67 positivity ranged from 5% to 30% in low-grade cases and from 50% to 70% in high-grade cases. Gene rearrangement analysis showed rearrangement with Jh probe and half of the cases were also rearranged with the Kde probe (Kappa constant chain gene). A rearrangement banding pattern with TCR genes was not present in any of the cases. Cytogenetic study showed complex alterations in high grade cases and a normal karyotype in low grade lymphomas. Only one case had rearrangement for the bcl-2 probe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Female
  • Gastric Mucosa / pathology
  • Gastrointestinal Neoplasms / genetics
  • Gastrointestinal Neoplasms / pathology*
  • Gastrointestinal Neoplasms / ultrastructure
  • Gene Rearrangement
  • Humans
  • Intestinal Mucosa / pathology
  • Lymphoid Tissue / pathology
  • Lymphoma / genetics
  • Lymphoma / pathology*
  • Lymphoma / ultrastructure
  • Male
  • Thyroid Neoplasms / genetics
  • Thyroid Neoplasms / pathology*
  • Thyroid Neoplasms / ultrastructure