Integration of chicken genomic resources to enable whole-genome sequencing

Cytogenet Genome Res. 2003;102(1-4):297-303. doi: 10.1159/000075766.

Abstract

Different genomic resources in chicken were integrated through the Wageningen chicken BAC library. First, a BAC anchor map was created by screening this library with two sets of markers: microsatellite markers from the consensus linkage map and markers created from BAC end sequencing in chromosome walking experiments. Second, HINdIII digestion fingerprints were created for all BACs of the Wageningen chicken BAC library. Third, cytogenetic positions of BACs were assigned by FISH. These integrated resources will facilitate further chromosome-walking experiments and whole-genome sequencing.

MeSH terms

  • Animals
  • Chickens / genetics*
  • Chromosome Mapping / veterinary
  • Chromosomes, Artificial, Bacterial / genetics
  • Contig Mapping / veterinary
  • Cytogenetic Analysis / veterinary
  • DNA / genetics
  • DNA Fingerprinting / veterinary
  • Genome*
  • Genomics / trends*
  • In Situ Hybridization, Fluorescence / veterinary
  • Microsatellite Repeats / genetics
  • Sequence Analysis, DNA / trends*
  • Sequence Analysis, DNA / veterinary*
  • Sequence Tagged Sites

Substances

  • DNA