Characterization of cytolethal distending toxin genes and expression in shiga toxin-producing Escherichia coli strains of non-O157 serogroups

Martina Bielaszewska; Marina Fell; Lilo Greune; Rita Prager; Angelika Fruth; Helmut Tschäpe; M Alexander Schmidt; Helge Karch

doi:10.1128/IAI.72.3.1812-1816.2004

Characterization of cytolethal distending toxin genes and expression in shiga toxin-producing Escherichia coli strains of non-O157 serogroups

Infect Immun. 2004 Mar;72(3):1812-6. doi: 10.1128/IAI.72.3.1812-1816.2004.

Authors

Martina Bielaszewska¹, Marina Fell, Lilo Greune, Rita Prager, Angelika Fruth, Helmut Tschäpe, M Alexander Schmidt, Helge Karch

Affiliation

¹ Institut für Hygiene, Universitätsklinikum Münster, Zentrum für Molekularbiologie der Entzündung (ZMBE), Universität Münster, 48149 Münster, Germany. [email protected]

Abstract

We identified cytolethal distending toxin and its gene (cdt) in 17 of 340 non-O157 Shiga toxin-producing Escherichia coli (STEC) strains (serotypes O73:H18, O91:H21, O113:H21, and O153:H18), all of which were eae negative. cdt is either chromosomal and homologous to cdt-V (serotypes O73:H18, O91:H21, and O113:H21) or plasmidborne and identical to cdt-III (serotype O153:H18). Among eae-negative STEC, cdt was associated with disease (P = 0.003).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Toxins / genetics*
Base Sequence
CHO Cells
Chromosome Mapping
Cricetinae
DNA, Bacterial / genetics
Escherichia coli / classification
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / microbiology
Gene Expression
Genes, Bacterial*
Humans
Molecular Sequence Data
Serotyping
Shiga Toxin / biosynthesis

Substances

Bacterial Toxins
DNA, Bacterial
cytolethal distending toxin
Shiga Toxin

Associated data

GENBANK/AY365042
GENBANK/AY365043
GENBANK/AY365044
GENBANK/AY365045