Abstract
The Arabidopsis genome has two similar dynamin-like proteins, ADL2a and ADL2b (76.7% identity). ADL2a is reported to be localized in chloroplasts [Kang et al. (1998) Plant Mol. Biol. 38: 437], while ADL2b functions in mitochondrial division [Arimura and Tsutsumi (2002) PROC: Natl. Acad. Sci. USA 99: 5727]. Using GFP fusion proteins, we observed both ADL2a and ADL2b in portions of mitochondria but not in chloroplasts. Furthermore, cells transformed with ADL2a and ADL2b with a defective GTPase domain had normal chloroplasts but elongated mitochondria. These results imply that both ADL2b and ADL2a are involved in the division of plant mitochondria.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence / genetics
-
Arabidopsis / genetics
-
Arabidopsis / growth & development*
-
Arabidopsis / metabolism*
-
Arabidopsis Proteins / genetics
-
Arabidopsis Proteins / metabolism*
-
Cell Division / genetics
-
Chloroplasts / genetics
-
Chloroplasts / metabolism
-
GTP Phosphohydrolases / genetics
-
GTP Phosphohydrolases / metabolism*
-
Green Fluorescent Proteins
-
Luminescent Proteins
-
Mitochondria / genetics
-
Mitochondria / metabolism*
-
Protein Structure, Tertiary / genetics
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
Substances
-
ADL2 protein, Arabidopsis
-
Arabidopsis Proteins
-
Luminescent Proteins
-
Recombinant Fusion Proteins
-
Green Fluorescent Proteins
-
ADL2b protein, Arabidopsis
-
GTP Phosphohydrolases