Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

FEBS Lett. 1992 Aug 17;308(2):161-4. doi: 10.1016/0014-5793(92)81266-o.

Abstract

A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Cricetinae
  • Cricetulus
  • DNA
  • Gene Expression
  • Glucuronosyltransferase*
  • Hexosyltransferases / genetics
  • Hexosyltransferases / metabolism*
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Lung / cytology
  • Lung / metabolism
  • Microsomes, Liver / enzymology
  • Plasmids
  • Substrate Specificity

Substances

  • Isoenzymes
  • bilirubin glucuronoside glucuronosyltransferase
  • DNA
  • Hexosyltransferases
  • Glucuronosyltransferase