Monoclonal antibodies against metallothioneins from the human liver

Immunol Lett. 1992 Mar;32(1):21-6. doi: 10.1016/0165-2478(92)90193-r.

Abstract

Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern when tested with MTs from the livers of different mammals. A double-antibody sandwich enzyme-linked-immunosorbent-assay (ELISA) was developed with the antibodies L2E3 and L5G2. This assay allows the detection of 60 pg human fetal liver MT and exhibits a metal dependent response for Zn-, Cd- and Hg-MT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Cross Reactions
  • Dogs / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Hybridomas / immunology
  • Kidney / chemistry
  • Liver / chemistry*
  • Liver / embryology
  • Metallothionein / classification
  • Metallothionein / immunology*
  • Metallothionein / isolation & purification
  • Mice
  • Mice, Inbred BALB C / immunology
  • Papio / immunology
  • Rabbits / immunology
  • Rats / immunology

Substances

  • Antibodies, Monoclonal
  • Metallothionein