Metalated hybrid polymers as catalytic reagents for phosphate ester hydrolysis and plasmid modification

Vadapalli Chandrasekhar; Pravas Deria; Venkatasubbaiah Krishnan; Arunachalampillai Athimoolam; Sanjay Singh; C Madhavaiah; S G Srivatsan; Sandeep Verma

doi:10.1016/j.bmcl.2003.12.082

Metalated hybrid polymers as catalytic reagents for phosphate ester hydrolysis and plasmid modification

Bioorg Med Chem Lett. 2004 Mar 22;14(6):1559-62. doi: 10.1016/j.bmcl.2003.12.082.

Authors

Vadapalli Chandrasekhar¹, Pravas Deria, Venkatasubbaiah Krishnan, Arunachalampillai Athimoolam, Sanjay Singh, C Madhavaiah, S G Srivatsan, Sandeep Verma

Affiliation

¹ Department of Chemistry, Indian Institute of Technology, Kanpur-208016, India. [email protected]

PMID: 15006403
DOI: 10.1016/j.bmcl.2003.12.082

Abstract

Pendant pyrazolylcyclophosphazene containing hybrid cross-linked polymer (CPPL) has been utilized for binding Zn(II). The metalated polymer (CPPL-Zn) has been found to be very effective catalyst for the hydrolysis of a RNA model phosphodiester substrate [2-(hydroxypropyl)-p-nitrophenyl phosphate, hNPP]. In addition, CPPL-Zn also cleaved supercoiled plasmid DNA pBR322 thus providing a novel structural motif of inorganic-organic hybrid polymers as synthetic nucleases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis / drug effects
Esters
Hydrolysis / drug effects
Metals / chemistry
Metals / pharmacokinetics*
Phosphates / chemistry
Phosphates / pharmacokinetics*
Plasmids / chemistry
Plasmids / pharmacokinetics*
Polymers / chemistry
Polymers / pharmacokinetics*

Substances

Esters
Metals
Phosphates
Polymers