Abstract
The PATCHED (PTC) gene is recognized as a tumor suppressor in basal cell carcinoma. Mapping of a minimal region of deletion at 9q22.3 and observation of a decreased PTC expression in superficial papillary bladder tumors led us to hypothesize that it could also be involved in this cancer. To further investigate this hypothesis, we submitted Ptc(+/-) heterozygous mutant mice and their wild-type littermates to chemical carcinogenesis by adding N-butyl-N-(4-hydroxybutyl) nitrosamine to their drinking water. Preneoplastic and neoplastic changes were observed significantly earlier in the Ptc(+/-) than in the wild-type mice. Our data support the hypothesis of Ptc acting as a tumor suppressor gene in bladder cancer.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Butylhydroxybutylnitrosamine / toxicity
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Carcinogens
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Carcinoma, Transitional Cell / chemically induced
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Carcinoma, Transitional Cell / genetics
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Cell Division
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Cocarcinogenesis
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Gene Deletion
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Genotype
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Heterozygote
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In Situ Nick-End Labeling
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Intracellular Signaling Peptides and Proteins
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Male
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Membrane Proteins / genetics*
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Mice
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Mice, Inbred C57BL
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Neoplasm Invasiveness / genetics
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Neoplasm Invasiveness / pathology
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Patched Receptors
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Patched-1 Receptor
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Receptors, Cell Surface
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Urinary Bladder / physiology
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Urinary Bladder Neoplasms / chemically induced
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Urinary Bladder Neoplasms / genetics*
Substances
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Carcinogens
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Patched Receptors
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Patched-1 Receptor
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Ptch1 protein, mouse
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Receptors, Cell Surface
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Butylhydroxybutylnitrosamine